Influence of NPs Ni° on the induction of oxidative damage in Triticum vulgare

The influence of spherical nanoparticles (NPs) of nickel Ni° (70±0.3 nm in diameter) in the concentrations of 0.025, 0.05 and 0.1 M on the root part of 4-day-old seedlings of wheat Triticum vulgare wheat has been studied. After treating plants with nanoparticles, an increase was observed in the fluorescence of dichlorofluorescein and malondialdehyde (MDA), as well as strong haphazard DNA degradation down to discrete fragments of flowing nucleotides without forming abnormal apoptotic “ladders”; these effects were directly dependent on the dosage. Fluorescence microscopy and assessment of cell viability by the number of dead cells showed an increase in plant cells death after increasing the number of NPs in the environment, primarily by the way of necrosis.